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(A) 3D midbrain organoid slice labeled <t>with</t> <t>tyrosine</t> hydroxylase, <t>NeuN</t> and Hoechst 33342 to demonstrate multicellularity of the organoids. (B) Image quantification of slice in (A) as evidence for NeuN- and NeuN+ cell types within the 3D culture. (C) Western blot results from organoids under starvation conditions and inhibition of autophagosome degradation. (D-F) Decomposition of GE(1) to show within-group and between-group effects. (G-I) GE(1) T within responses for nuclei, TUJ1+ neurons, and GFAP+ astrocytes, respectively. (J-L) Decomposition of GE(0) to show within-group and between-group effects. (M-O) GE(0) L within responses for nuclei, TUJ1+ neurons, and GFAP+ astrocytes, respectively. * = p<0.05, ** = p<0.01, *** = p<0.001, **** = p<0.0001. All statistics are from ANOVA with multiple comparisons post hoc. Each point represents 1 FOV. N=3 organoids.
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(A) 3D midbrain organoid slice labeled <t>with</t> <t>tyrosine</t> hydroxylase, <t>NeuN</t> and Hoechst 33342 to demonstrate multicellularity of the organoids. (B) Image quantification of slice in (A) as evidence for NeuN- and NeuN+ cell types within the 3D culture. (C) Western blot results from organoids under starvation conditions and inhibition of autophagosome degradation. (D-F) Decomposition of GE(1) to show within-group and between-group effects. (G-I) GE(1) T within responses for nuclei, TUJ1+ neurons, and GFAP+ astrocytes, respectively. (J-L) Decomposition of GE(0) to show within-group and between-group effects. (M-O) GE(0) L within responses for nuclei, TUJ1+ neurons, and GFAP+ astrocytes, respectively. * = p<0.05, ** = p<0.01, *** = p<0.001, **** = p<0.0001. All statistics are from ANOVA with multiple comparisons post hoc. Each point represents 1 FOV. N=3 organoids.
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(A) 3D midbrain organoid slice labeled with tyrosine hydroxylase, NeuN and Hoechst 33342 to demonstrate multicellularity of the organoids. (B) Image quantification of slice in (A) as evidence for NeuN- and NeuN+ cell types within the 3D culture. (C) Western blot results from organoids under starvation conditions and inhibition of autophagosome degradation. (D-F) Decomposition of GE(1) to show within-group and between-group effects. (G-I) GE(1) T within responses for nuclei, TUJ1+ neurons, and GFAP+ astrocytes, respectively. (J-L) Decomposition of GE(0) to show within-group and between-group effects. (M-O) GE(0) L within responses for nuclei, TUJ1+ neurons, and GFAP+ astrocytes, respectively. * = p<0.05, ** = p<0.01, *** = p<0.001, **** = p<0.0001. All statistics are from ANOVA with multiple comparisons post hoc. Each point represents 1 FOV. N=3 organoids.

Journal: bioRxiv

Article Title: Dispersion indices for universal quantification of fluorescently-labelled subcellular structure spatial distributions

doi: 10.1101/2024.08.18.608451

Figure Lengend Snippet: (A) 3D midbrain organoid slice labeled with tyrosine hydroxylase, NeuN and Hoechst 33342 to demonstrate multicellularity of the organoids. (B) Image quantification of slice in (A) as evidence for NeuN- and NeuN+ cell types within the 3D culture. (C) Western blot results from organoids under starvation conditions and inhibition of autophagosome degradation. (D-F) Decomposition of GE(1) to show within-group and between-group effects. (G-I) GE(1) T within responses for nuclei, TUJ1+ neurons, and GFAP+ astrocytes, respectively. (J-L) Decomposition of GE(0) to show within-group and between-group effects. (M-O) GE(0) L within responses for nuclei, TUJ1+ neurons, and GFAP+ astrocytes, respectively. * = p<0.05, ** = p<0.01, *** = p<0.001, **** = p<0.0001. All statistics are from ANOVA with multiple comparisons post hoc. Each point represents 1 FOV. N=3 organoids.

Article Snippet: Neurons were identified with Tyrosine Hydroxylase (1:1000 Abcam, ab112), Neuronal marker NeuN (1:500, Abcam, ab177487), and TUJ1 (1:1000, Sigma-Aldrich, MAB1637).

Techniques: Labeling, Western Blot, Inhibition